To use an IR spectrum table, first find the frequency or compound in the first column, depending on which type of chart you are using. The melting point range can be indicated and recorded with the help of a thermometer. Near infrared transmittance spectroscopy was used to determine the analgesic paracetamol in a pharmaceutical preparation commercially available as tablets. for each of the four IR spectra shown. Paracetamol or acetaminophen is a widely used over-the-counter analgesic (pain reliever) SpectraBase Spectrum ID: 67jwNuR3vBi: Name: Acetaminophen: Source of Sample: Sigma-Aldrich Inc. (but don't forget about part 2) Part 2 IR spectra of solid samples. 2022-05-28. These spectra were obtained from an Nanalysis 60 PRO ( 1 H- 13 C) 4-Hydroxypropiophenone. Specific bands may fall over a range of wavenumbers, cm-1. Go To: Top, References, Notes Data compilation copyrightby the U.S. Secretary of Commerce on behalf of the U.S.A.All rights reserved. The infrared absorption spectrum is concordant with the spectrum obtained from paracetamol R or with the reference spectrum of paracetamol. Comtrex tablets composed of paracetamol, pseudoephedrine and brompheniramine are widely used for relieving symptoms related to common cold. The functional groups in acetaminophen are hydroxyl, aromatic ring, and amide. Infrared spectroscopy (IR spectroscopy or vibrational spectroscopy) is the measurement of the interaction of infrared radiation with matter by absorption, emission, or reflection.It is used to study and identify chemical substances or functional groups in solid, liquid, or gaseous forms. Paracetamol, with a 13CH3 in the acetyl group (paracetamol-13CH3) was also synthesized and dosed to rats i.p. ChEBI. The infrared absorption spectrum of this product should be consistent with that of the control (Spectrum set 131). In general, spectroscopy is the study of the interaction between light and matter. The analysis section has 5 parts: Part 1 calculating the percentage yield of paracetamol. The analysis section has 5 parts: Part 1 calculating the percentage yield of paracetamol. Bonds in organic molecules vibrate energies in theinfrared region of the electromagnetic spectrum and eachtype of bond has a distinct region of the spectrum inwhich it vibrates. Paracetamol will absorb infraredradiation related to the energies of the bonds itpossesses. This study has overcome the challenging dosage form ratio (250:15:1) and proposed chromatographic methods for analyzing the ternary combination were utilized displaying different apparatus, solvents and 4 This is only a general 'rule of thumb' but it is useful because it can be difficult to determine whether a carbon atom has 'gained' or 'lost' electrons. Spectroscopy methods [1,2] Near infrared: 0.75- 2.2 m Mid infrared: 2.5-50 m Far infrared: 50-1000 m: Table 1: Regions of electromagnetic spectrum. Last Update2022-01-01 11:42:57. These spectra show distinct differences, especially for the resonances arising from the aromatic carbons, i.e., between 115 and 125 ppm. The 13 C CP/MAS solid-state NMR spectrum of commercial paracetamol is in agreement with that reported previously, but it is more resolved. The 1 H NMR spectrum of a 4% (w/w; 330 mM) solution of aspirin in chloroform-D was measured at 82 MHz using the Thermo Scientific Ibuprofen. Infrared spectroscopy is a particular technique that can be used to help identify organic (carbon-based) compounds. The NH stretches of amines are in the region 3300-3000 cm -1. substancial - Free ebook download as Text File (.txt), PDF File (.pdf) or read book online for free. recrystallized product using IR analysis. SpectraBase Spectrum ID: uvbq6dsvv3: Name: ACETAMINOPHEN: Source of Sample: S. B. Penick & Company: CAS Registry Number: 103-90-2: In order to maintain the optimum level of caffeine, various spectrophotometric methods have been developed. of and in " a to was is ) ( for as on by he with 's that at from his it an were are which this also be has or : had first one their its new after but who not they have The aim of this work was to use a spectrophotometric method for thedetermination of paracetamol in pharmaceutical formulations.The quantification of paracetamol through infrared spectroscopy was developed and validated for pharmaceuticals in tablet forms. B. The IR spectrum table and chart are provided via a web-based graphical user interface (GUI). Acetaminophen. Fourier Transform Infrared (FTIR) spectroscopy is a technique used to determine qualitative and quantitative features of IR-active molecules in organic or inorganic solid, liquid or gas samples. An introduction to the IR spectroscopy of the carbonyl group, exploring why the peak is intense and showing how to apply that knowledge to the analysis of the spectra of ketones. INFRARED SPECTROSCOPY (IR) Theory and Interpretation of IR spectra ASSIGNED READINGS Introduction to technique 25 (p. 833-834 in lab textbook) Uses of the Infrared Spectrum (p. 847-853) Look over pages 853-866 after viewing this presentation for additional examples of various functional groups. This is the laboratory report the exploration of the factors influencing nucleophilic substitution and consideration of the williamson synthesis abstract 4-Aminophenol is a natural product found in Camellia oleifera with data available. For instance, the melting point of paracetamol ranges from168 to172 degree Celsius Infra-red spectroscopy is one of the most essentially useful methods for identification of compound. The IR spectra were collected from 4000 to 600 cm-1, with 10 scans per spectrum and 4.00 cm-1 resolution. Answer to Solved Label the IR spectra below of paracetamol (in. Beauchamp Spectroscopy Tables 1 Z:\classes\spectroscopy\all spectra tables for web.DOC Infrared Tables (short summary of common absorption frequencies) The values given in the tables that follow are typical values. Create. Principles (design and approach) The infra-red spectrum (Fig. 1137 Projects 1137 incoming 1137 knowledgeable 1137 meanings 1137 1136 demonstrations 1136 escaped 1136 notification 1136 FAIR 1136 Hmm 1136 CrossRef 1135 arrange 1135 LP 1135 forty 1135 suburban 1135 GW 1135 herein 1135 intriguing 1134 Move 1134 Reynolds 1134 positioned 1134 didnt 1134 int 1133 Chamber 1133 termination 1133 overlapping 1132 newborn 1132 Publishers 1132 Turn in the duplicate pages of your notebook at the end of the lab period, and you're done. Specificity. Three accurate, simple and sensitive spectrophotometric methods were developed for the simultaneous determination of Paracetamol and Ibuprofen in Mean of two measurements, Loss = 0.13% IDENTIFICATION TESTS INFRARED SPECTROPHOTOMETERY (Comparative identification analysis demonstrates direct Note that not all frequencies have a related compound. 2004-09-16. The spectra produce a profile of the sample, a distinctive molecular fingerprint that can be used to screen and scan samples for many different components. IR spectra of the starting material salicylic acid and the product acetosalicylic acid (aspirin) are attached. Spectrum type: 1 H NMR Spectrum (1D, 500 MHz, 5%_DMSO, experimental) Disclaimer: While we have made our best-effort to label most spectral peaks, certain spectral features may not be fully annotated in Hot Network Questions Are the computers on Pioneer 10 & 11 still running? Deciphering 1H-NMR Spectra. This experiment focused on the determination of the Paracetamol content of Biogesic, and to assess the Percentage Label Claim of the branded Paracetamol Tablet, in comparison to a USP Grade Paracetamol Standard. Spectroscopy methods [1,2] Near infrared: 0.75- 2.2 m Mid infrared: 2.5-50 m Far infrared: 50-1000 m: Table 1: Regions of electromagnetic spectrum. Transcribed image text: %Transmittance 4 01- 5 6 701 8 901 3800 funnym paracetamol 20200326 3600 3400 3320.395 3200 3155.877 3107.623 3000 2800 2792.032 2600 2587.417 2400 Wavenumber 2342.166 2200 2112.090 2000 1800 1600 1649.646 1608.763 1560.233 1504.705 1431.813 IR spectra of paracetamol have been measured for powder crystals. Contamination with traces of para -aminophenol, and humid conditions that cause hydrolysis to para -aminophenol, result in further degradation and discoloration. In this article, we will summarize the concept of proton NMR, the most common NMR information acquired by organic chemists. This makes it a good technique to investigate functional groups such as the O-H in alcohols, the C=O in carboxylic acids, etc. Method Validation 3.3.1. 1-aminobutane. 2.1 Materials and Reagents That double trough (typical of primary amines) can be seen One of the most important concepts taught in organic chemistry is the method for determining the chemical structure of newly synthesized or unknown compounds. Carry out the examination with the residue as described under 1.7 Spectrophotometry in the infrared region. 1 Paracetamol was marketed as Panadol by Sterling-Winthrop Co in 1953 in the UK. It can be used to characterize new materials or identify and verify known and unknown samples. Labelled IR spectrum of N-(4-butoxyphenyl)acetamide produce in featured in Results Section 3.3.Figure 1. Specific substituents may cause variations in absorption frequencies. Phenacetin (C 10 H 13 NO 2 ), N- (4-Ethoxyphenyl)acetamide, is a drug that saw widespread application for nearly a century in many over-the-counter medications as a remedy for pain and fever. Heating of supercooled amorphous paracetamol from 295 K in a covered sample under IR bands in the 1650-1700 cm ~ region. 3 At 25 o C pKa = 9.51. In comparing the IR spectrums, it is apparent of the absence of the O-H stretch in the product as opposed to the broad O-H stretch shown between 3200-3400 cm-1 in the IR spectra of the pure paracetamol. Paracetamol infrared spectrum issues. UNK the , . 4-aminophenol is an amino phenol (one of the three possible isomers) which has the single amino substituent located para to the phenolic -OH group. Diethyl phthalate. A-level Spectroscopy. > A functional group is a specific group of atoms within a molecule that gives rise to the characteristic chemical reactions of the molecule. The peak at 151 can be accounted for the unfragmented molecule and 43 for a C2H3O group but what about the rest. 2004-09-16. Compound name: Acetaminophen. Use past-passive tense. Region in an electromagnetic spectrum: A) Visible B) Infrared C) Ultraviolet D) All of the above. Catalog Number: A7085: Lot Number: 099K0126: Accessory: 1) of caffeine dissolved in chloroform shows two characteristic bands at 1655 and 1700 cm ~. IR Spectroscopy Tutorial: Amines. Shake the tablet powder sample with ca 150 ml of acetic acid (0.05 M) for 5 min in a 250 ml volumetric flask and then adjust the volume to 250 ml with more acetic acid (0.05 M). Introduction. An image of a human brain from a live patient recorded using magnetic resonance imaging - a 3D form of n.m.r. For the case of paracetamol, we show how terahertz time-domain spectroscopy can be used to characterize the solid and liquid phase dynamics. Explain how planes in a lattice are labelled. at 40 mg/kg (N = 3). The engine is presently applied to generate the IR spectra available on the web, targeting billions of pure substance IR spectra to be created over the next few years. It has a role as a metabolite and an allergen. You may experience the IR spectra for 20 sample substances prior to purchasing. Figure : Paracetamol. Paracetamol is a very popular medication used to treat pain and fever. Paracetamol is an analgesic-antipyretic drug and Ibuprofen is a non-steroidal anti-inflammatory drug. But an "OH" group It is a common ingredient in a variety of drinks (soft and energy drinks) and is also used in combination with various medicines. How many samples should be tested if a batch consists of 120 bottles of paracetamol suspension when the square root method is used? 5. Infrared spectroscopy is the method described by the pharmacopoeias to identify paracetamol, the present investigation shows the development and validation, according to the US Pharmacopoeia recommendation of the quantitative analysis of paracetamol in tablets through infrared spectroscopy.The results allowed to improve that a standard The analgesic paracetamol is also available in a range of dosage forms and strengths to meet the specific needs of the user, including tablets, dispersible tablets, paediatric soluble tablets, paediatric oral solution, sugar-free oral solution, oral suspension, doublestrength oral suspension and suppositories. Ref. The remaining Raman scattered light is then passed into a spectrograph and captured using a CCD detector to give a Raman spectrum like that shown in Figure 11 of paracetamol. Cholesterol. This study has overcome the challenging dosage form ratio (250:15:1) and proposed chromatographic methods for analyzing the ternary combination were utilized displaying different apparatus, solvents and The two substances have many structural features in common, resulting in similar peaks of their spectra. double bond or aromatic ring. Transmission Infrared (IR) Spectrum. Chemicals and reagents. To generate the IR spectrum, different frequencies of infrared light are passed through a sample, and the transmittance of light at each frequency is measured. Regions of the IR spectrum 7 . Regions of the IR spectrum 8 . IR spectra of paracetamol and phenacetin have been measured for powder crystals of these compounds and for their solutions in chloroform and dimethylsulfoxide. Ab initio calculations of their equilibrium geometry and vibrational spectra were carried out for spectrum interpretation. 4-Aminophenol is a natural product found in Camellia oleifera with data available. ' '' ''' - -- --- ---- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- ----- A) 12 B) 11 C) 10 D) 9. 2.0 Methodology Use the number format in separating procedures. Primary amines contain the -NH 2 group, and so have N-H bonds. The absorption band N-H stretching at 3159.18 cm 1 and C=O stretching at 1655.92 cm 1 appeared in the spectrum of the physical mixture of paracetamol and Acacia etbaica. Eugenol. It is tempting to call it an alcohol group. Surface-enhanced Raman spectroscopy (SERS) is a highly sensitive technique that enhances the Raman scattering of molecules supported by some nanostructured materials. The solvent does not have an interference band in the 1600-1700cm -~ region. 3.3. FT-IR spectra of paracetamol standards. Characterization by IR Spectroscopy. Analysis of Paracetamol (Afternoon) In the afternoon you will analyse the purity of the paracetamol you have made and interpret the infrared (IR) and nuclear magnetic resonance (NMR) spectra of paracetamol to confirm you have made the correct compound. 6. The spectrum attained from IR spectroscopy is below: Figure 2.2 The IR spectrum of synthesized aspirin displays two peaks in the 1650 cm(^-1) to 1850 cm(^-1) range at 1679.70 cm(^-1) and at 1749.46 cm(^-1) Raman spectroscopy, in particular, has been applied exten-sively in the characterization and identification of suspected medicines [34, 36, 37] and also in the quantification of paracetamol [38]. 1 Paracetamol was marketed as Panadol by Sterling-Winthrop Co in 1953 in the UK. spectroscopy. The infrared absorption spectrum of this product should be consistent with that of the control (Spectrum set 131). See the test described below under "Related substances". The 13 C CP/MAS solid-state NMR spectrum of commercial paracetamol is in agreement with that reported previously, but it is more resolved. 2 Paracetamol was marketed as Tylenol by McNeil Laboratories in 1955 in the USA. These bands are weaker and sharper than those of the alcohol OH stretches which appear in the same region. A) 12. As shown in Figure 4(c), the OH stretching band of paracetamol at 3322.44 cm 1 was also observed in the spectrum of its mixture with the gum. Mid-infrared (MIR) spectroscopy is a powerful tool for characterising the vibrations of molecular bonds and is therefore ideal for label-free detection of Then find the corresponding values for absorption, appearance and other attributes. Shake the tablet powder sample with ca 150 ml of acetic acid (0.05 M) for 5 min in a 250 ml volumetric flask and then adjust the volume to 250 ml with more acetic acid (0.05 M). Weigh out tablet powder containing 125 mg 10 mg of paracetamol. The spectrum of a model mixture of materials, for a paracetamol tablet containing 80% paracetamol, 10% maize starch, 5% microcrystalline cellulose and 5% magnesium stearate is shown in Fig 2C. Weigh out tablet powder containing 125 mg 10 mg of paracetamol. A. The infra-red spectrum for a primary amine. The full spectrum can only be viewed using a FREE account. View the Full Spectrum for FREE! : Paracetamol; IP ASSAY vs. IP REFERENCE STANDARD (as is basis) ASSAY VALUE 99.4% vs. IP LOT IPRS/54/14 Labeled Content= 99.5% w/w on as is basis LOSS ON DRYING/VOLATILES Method: Dry in an oven. The laser light is scattered off the sample and passed through a filter to remove the much more intense Rayleigh scattered component. 5. This is a very useful tool for interpreting IR spectra: Only alkenes and aromatics show a CH stretch slightly higher than 3000 cm -1. The structure of acetaminophen is The group at the top of the molecule is a hydroxyl group. contains some random words for machine learning natural language processing Spectroscopy is a collective name for the various techniques that use the interaction between molecules and electromagnetic radiation to elucidate the structure of molecules. D) All of the above. Aspirin (C 9 H 8 O 4), acetylsalicylic acid, is a salicylate drug used to treat a variety of conditions, including headaches, fever and inflammation.It is also used in low doses in preventive treatment against heart attacks, strokes and blood clots. The infrared spectra within each image were collected at 8 cm 1 resolution in the 3000900 cm 1 the SORS spectra are compared with reference spectra of talc, paracetamol and calcium spectrum (see discussion in the next paragraph). 4.Carbonyl peaks can be shifted to lower wavenumbers when the carbonyl function is conjugated to a p -system e.g. Comtrex tablets composed of paracetamol, pseudoephedrine and brompheniramine are widely used for relieving symptoms related to common cold. 2 Paracetamol was marketed as Tylenol by McNeil Laboratories in 1955 in the USA. HMDB ID: HMDB0001859. The spectrum of a model mixture of materials, for a paracetamol tablet containing 80% paracetamol, 10% maize starch, 5% microcrystalline cellulose and 5% magnesium stearate is shown in Fig 2C. 3.2. The value for absorption is usually in cm -1. 2022-05-28. The nature of caffeine reveals that it is a bitter white crystalline alkaloid. Abstract. Panadol night tablets (Batch No. With in-depth features, Expatica brings the international community closer together. This product contains paracetamol (C8H9NO2) should be labeled the amount of 95.0% to 105.0%. The NMR spectra were collected using picoSpin 45 and 80 NMR spectrometers. As being mentioned above, within the wavenumber zone from 1800 cm 1 to 1300 cm 1, the infrared spectra of mixtures containing Siloflam tablet powder and paracetamol depended mostly on the infrared absorbance of sildenafil citrate and paracetamol (see also Figures 1(a) and 1(b)).Thanks to the specificity of infrared spectra of A very simple, non-destructive, inexpensive and green strategy was applied for the simultaneous determination of ibu-profen (IBP) and paracetamol (PC) using transmission Fourier Transform Infrared (FTIR) spectroscopy in tablet formulations for routine quality control laboratories. Use past-passive tense. 3 At 25 o C pKa = 9.51. A must-read for English-speaking expatriates and internationals across Europe, Expatica provides a tailored local news service and essential information on living, working, and moving to your country of choice. It has a role as a metabolite and an allergen. The table at right shows IR Create. 124992) labeled to contain 500 mg of Paracetamol and 25 mg of Diphenhydramine Hydrochloride , manufactured by GlaxoSmithKline company . trait . The monitoring of caffeine is very important Spectra were recorded on a dedicated instrument that measures the transmission of intact tablets over the wavelength range 6001900 nm. ! in the IR spectrum of NOD which is almost absent in the IR spectrum of DH is an evidence of the oxidation to ChEBI. Carvone. Last Update2022-01-01 11:42:57. A set of paracetamol tablets purchased in Malaysian pharmacies were compared to a similar set of sample purchased in the UK using near-infrared spectroscopy (NIRS). It is a rapid and relatively inexpensive method for the analysis of solids that are crystalline, Visible light is just a portion of the electromagnetic spectrum, and its the infrared section of the spectrum thats utilised in this technique. These bands are weaker and sharper than those of the alcohol OH stretches which appear in the same region. Figure 2.1 The NMR spectrum of synthesized aspirin displays a peak 2.4 PPM and a range of peaks from 7 PPM to 8.3 PPM. In addition to the Thermodynamics Research Center (TRC) data available from this site, much more physical and chemical property data is available from the following TRC products: View the Full Spectrum for FREE! The n (OH) peaks for acids and alcohols look very different and can be easily identified by looking at them (see reader). Simple Beers law calibration model Table 1 shows the response of the univariate simple Beers law calibration model in different regions of the spectra by applying different measurement criteria like peak height, peak area, baseline correction either with two points or without baseline correction. Figure : Paracetamol. Stability : Dry, pure paracetamol is stable to 45C. Additional samples of products containing ibuprofen or paracetamol in combination with other actives were added to the study as negative controls. Functional isomers in IR C 4 H 8 O 6 . Enter the email address you signed up with and we'll email you a reset link. View the Full Spectrum for FREE! Furthermore, near infrared spectroscopy (NIR) has been applied in this regard and in combination with Raman spectroscopy for authentication of They are co-formulated as tablets to improve analgesia, to simplify prescribing and to improve patient compliance. The metabolism and excretion of the 2H-labelled compound was followed in the rat using 600 MHz 1H and 92.1 MHz 2H NMR spectroscopy of urine collected 0-8, 8-24, 24-32 and 32-48 hr post-dosing. Brucine. But an "OH" group Figure 11: Raman spectrum of paracetamol. CoNLL17 Skipgram Terms - Free ebook download as Text File (.txt), PDF File (.pdf) or read book online for free. The n (OH) peaks for acids and alcohols look very different and can be easily identified by looking at them (see reader). Data compiled by: Structure, properties, spectra, suppliers and links for: phenacetin, 62-44-2, 40674-52-0. > A functional group is a specific group of atoms within a molecule that gives rise to the characteristic chemical reactions of the molecule. I know fragmentation will occur but i cannot account for all of them. 4 This is only a general 'rule of thumb' but it is useful because it can be difficult to determine whether a carbon atom has 'gained' or 'lost' electrons. View the Full Spectrum for FREE! To generate the IR spectrum, different frequencies of infrared light are passed through a sample, and the transmittance of light at each frequency is measured. The NH stretches of amines are in the region 3300-3000 cm -1. In primary amines (RNH 2 ), there are two bands in this region, the asymmetrical NH stretch and the symmetrical NH stretch. Analysis of Paracetamol (Afternoon) In the afternoon you will analyse the purity of the paracetamol you have made and interpret the infrared (IR) and nuclear magnetic resonance (NMR) spectra of paracetamol to confirm you have made the correct compound. double bond or aromatic ring. Nitro groups show two intense peaks in the IR spectrum: one between 1300-1400 cm-1 for the symmetric stretching mode, the other one between 1500-1600 cm-1 for the asymmetric stretching mode. 4-aminophenol is an amino phenol (one of the three possible isomers) which has the single amino substituent located para to the phenolic -OH group. The ball and stick molecule can be rotated. The 1 H NMR spectrum of a 4% (w/w; 330 mM) solution of aspirin in chloroform-D was measured at 82 MHz using the Thermo Scientific Paracetamol or acetaminophen is a widely used over-the-counter analgesic (pain reliever) Jump to main content Jump to site nav. Fourier Transform Infrared Spectroscopy (FTIR) identifies chemical bonds in a molecule by producing an infrared absorption spectrum. Functional groups are the portions in an organic molecule that dictate how the molecule will react. These absorb somewhere between 3100 and 3500 cm-1. Infrared Spectrum of Paracetamol : Click on the peaks in the spectrum to see the molecular vibration they corresponds to. [246], infrared spectroscopy [247] liquid chromatography [248], and HPLC methods [249,250] [249] [250]. Compounds that do not have a C=C bond show CH stretches only below 3000 cm -1. Melting point analysis is also useful for identification of compound. 2.0Methodology Use the number format in separating procedures. Spectrum Details. This experiment focused on the determination of the Paracetamol content of Biogesic, and to assess the Percentage Label Claim of the branded Paracetamol Tablet, in comparison to a USP Grade Paracetamol Standard. It is tempting to call it an alcohol group. NMR Spectrum of Phenacetin. 2.1.3. The full spectrum can only be viewed using a FREE account. This product contains paracetamol (C8H9NO2) should be labeled the amount of 95.0% to 105.0%. IR Spectroscopy Tutorial: Amines. The structure of acetaminophen is The group at the top of the molecule is a hydroxyl group. Attenuated Total Reflectance Infrared (ATR-IR) Spectrum. As you learned in an earlier experiment, infrared (IR) radiation interacts with the bonds between the atoms of a molecule. The functional groups in acetaminophen are hydroxyl, aromatic ring, and amide. All vibrational modes are included in both the stretching and bending regions. Expatica is the international communitys online home away from home. Appearance of IR Spectrum Wavelenght -= 10 6 meter Wavenumber (frequency) cm-1 = Hz /c 98% transmission 3% transmission 5 . 4.Carbonyl peaks can be shifted to lower wavenumbers when the carbonyl function is conjugated to a p -system e.g. At the moment I am looking at a mass spectrum for paracetemol (4-Hydroxy- (N-ethanoyl-aminobenzene)) which has four main m/z peaks at around 43, 80, 110 and 151. trait . Functional groups are the portions in an organic molecule that dictate how the molecule will react. In primary amines (RNH 2 ), there are two bands in this region, the asymmetrical NH stretch and the symmetrical NH stretch. Spectroscopy data: Infrared, ultraviolet, nuclear magnetic resonance, fluorescence and mass spectra have been reported. The IR spectra were collected using a Nicolet iS5 FT-IR spectrometer with an iD5 single bounce ATR accessory equipped with a diamond laminate crystal. Preparation of beverage samples 2. 2.1Materials and Reagents For the determination of the active pharmaceutical ingredients (API), KBr pellets containing known Aspirin (C 9 H 8 O 4), acetylsalicylic acid, is a salicylate drug used to treat a variety of conditions, including headaches, fever and inflammation.It is also used in low doses in preventive treatment against heart attacks, strokes and blood clots.